Dye Industry
To observe microscopic prokaryotes and eukaryotes, the blots were sought to improve its visibility. The development of these colorings were driven by chemical absorption of dye required in the textile industry since 1850.
Malachowski and Romanowski used acid and basic dyes, which gave rise to Geimsa, Leishman, MacNeal and Wright staining to identify parasites in blood cells and hematopoietic cells for identification.
Between 1960 and 1970 the onset of flow cytometry directed the development of several new dyes, and the measurement of DNA content has become one of the first major application of this technique
All dyes mentioned above are used frequently in Flow Cytometry
Meanwhile, in 1940, Coons, Creech and Jones used antipneumococcal antibodies conjugated anthracene to detect microorganisms in tissues.
Shapiro HM. Practical flow cytometry. 4th ed. New York: Wiley-Liss; 2003
Malachowski and Romanowski used acid and basic dyes, which gave rise to Geimsa, Leishman, MacNeal and Wright staining to identify parasites in blood cells and hematopoietic cells for identification.
- 1880 - The fluorescent dyes had not appeared until the fluorescein was synthesized and used by Paul Ehrlich, who also pioneered the use of mixtures of acid and basic dyes to understand the internal structures of leukocytes.
- 1900 - DNA dyes were used for the first time.
Between 1960 and 1970 the onset of flow cytometry directed the development of several new dyes, and the measurement of DNA content has become one of the first major application of this technique
- 1969 - Dittrich and Gohde were the first to apply ethidium bromide
- 1973 - Crissman and Steinkamp introduced Propidium
- 1974 - Crissman and Tobey introduced Mithramycin
- 1976 - Latt and Stetten started using the dyes Hoechst
- 1977 - Stohr introduced DAPI.
All dyes mentioned above are used frequently in Flow Cytometry
Meanwhile, in 1940, Coons, Creech and Jones used antipneumococcal antibodies conjugated anthracene to detect microorganisms in tissues.
- 1950 - using antibodies were conjugated to fluorescein isothiocyanate - FITC, creating the immunophenotyping (which identified the cells from the single set of membrane proteins).
Shapiro HM. Practical flow cytometry. 4th ed. New York: Wiley-Liss; 2003